HA-Tag Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86631A
Western blotting with Anti-HA rabbit polyclonal antibody at dilution of 1:1000. Lane1: HA tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with the HA-GFP, using anti-HA-Tag Polyclonal Antibody at 1:800 dilution.
IP Result of293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag rabbit polyclonal antibody. lane 1: rabbit IgG Isotype Control,Lane 2:input, Lane 3: anti-HA-Tag rabbit antibody
1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag pAb(RD86631A) and AF647 conjugated Goat Anti-Rabbit IgG (H+L);lsotype Control stained with 0.2ug Rabbit IgG and AF647 conjugated Goat Anti-Rabbit IgG (H+L)
Product Name
HA-Tag Polyclonal Antibody
Catalog Number
RD86631A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to int
Immunogen Information
Immunogen
Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
Synonyms
HA epitope tagHA1HA2Hemagglutinin HA1 chainHemagglutinin HA2 chain
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
All
Tested Applications
WB, IF,IP,FCM
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000, IP 3ug/sample, IF 1:800-1600,FCM:0.2 ug
Concentration
4mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.