HCAR3 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD89072A
Western blot analysis of extracts of Rat pancreas using HCAR3 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat pancreas using HCAR3 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse spleen using HCAR3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse spleen using HCAR3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat spleen using HCAR3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat spleen using HCAR3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human spleen using HCAR3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human spleen using HCAR3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Overview

Circle
Product Name
HCAR3 Polyclonal Antibody
Catalog Number
RD89072A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.

Immunogen Information

Circle
Immunogen
A synthetic peptide of human HCAR3
Gene ID
8843
Swissprot
P49019
Synonyms
GPR109BHCA3HM74PUMAGPuma-gHCAR3
Calculated MW
39kDa/40kDa/42kDa/44kDa
Observed MW
41kDa/43kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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