HDAC3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80997A
Western blot analysis of extracts of various cell lines using HDAC3 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of Mouse brain using HDAC3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse liver using HDAC3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using HDAC3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon carcinoma using HDAC3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using HDAC3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
HDAC3 Polyclonal Antibody
Catalog Number
RD80997A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
Immunogen Information
Immunogen
A synthetic peptide of human HDAC3 (NP_003874.2).
Gene ID
8841
Swissprot
O15379
Synonyms
HD3RPD3RPD3-2HDAC3
Calculated MW
48 kDa/49 kDa
Observed MW
48 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.