HIBADH Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89382A
Western blot analysis of extracts of various cell lines using HIBADH Polyclonal Antibody at 1:3000 dilution.
Immunohistochemistry of paraffin-embedded Human lung cancer using HIBADH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human esophageal using HIBADH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using HIBADH Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using HIBADH Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using HIBADH Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
HIBADH Polyclonal Antibody
Catalog Number
RD89382A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.
Immunogen Information
Immunogen
Recombinant fusion protein of human HIBADH
Gene ID
11112
Swissprot
P31937
Synonyms
HIBADHNS5ATP13-hydroxyisobutyrate dehydrogenase
Calculated MW
38kDa/39kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas