HIF1AN Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD77085A
Western Blot analysis of Mouse brain and liver tissue using HIF1AN Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human esophagus cancer using HIF1AN Polyclonal Antibody at dilution of 1:40
Immunohistochemistry of paraffin-embedded Human brain using HIF1AN Polyclonal Antibody at dilution of 1:40
Variation
Quantity
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Overview
Product Name
HIF1AN Polyclonal Antibody
Catalog Number
RD77085A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
Immunogen Information
Immunogen
Synthetic peptide of human HIF1AN
Swissprot
Q9NWT6
Synonyms
DKFZp762F1811Factor inhibiting HIF-1Factor inhibiting HIF1FIH 1FIH-1FIH1FLJ20615FLJ22027HIF1ANHIF1NHypoxia inducible factor 1 alpha inhibitorHypoxia inducible factor 1 alpha subunit inhibitorHypoxia inducible factor asparagine hydroxylaseHyp
Calculated MW
40 kDa
Gene Accession
NP_060372
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:300
Concentration
0.6 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas