HIF3A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using HIF3A Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using HIF3A Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
HIF3A Polyclonal Antibody
Catalog Number
RD89206A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
Immunogen Information
Immunogen
Recombinant fusion protein of human HIF3A
Gene ID
64344
Swissprot
Q9Y2N7
Synonyms
HIF-3AHIF3-alpha-1IPASMOP7PASD7bHLHe17
Calculated MW
9kDa/25kDa/50kDa/102kDa/306-860kDa
Observed MW
55KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.