His-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86705A
Western blot of His-Tag fused recombinant protein with Anti-His mouse monoclonal antibody at dilution of 1:1000.
IP Result of 293F cells transfected with His-Tag fusion protein,using anti-His-Tag mouse monoclonal antibody . Lane 1:input, Lane 2: anti-His-Tag mouse monoclonal antibody, Lane 3: mouse IgG1 Isotype Control
1x10^6 CHO cells Transfected with a His plasmid were stained with 0.2ug Anti-His tag mAb(RD86705A) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
Product Name
His-Tag Monoclonal Antibody
Catalog Number
RD86705A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.
Immunogen Information
Immunogen
Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.
Synonyms
6 His epitope tagHexa His tagHHHHHH epitope tagHHHHHH tagHis tagPolyhistidine Tag
Calculated MW
40kDa
Observed MW
40kDa
Applications
Reactivity
All
Tested Applications
WB,IP,FCM
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IP 3ug/sample,FCM:0.2 ug
Concentration
1mg/mL
Storage Buffer
PBS with 0.1% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.