His-Tag Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86615A
Western blot with anti-6xHis antibody at dilution of 1:2000.
lane 1: HEK 293 whole cell lysate, lane 2: 6xHis tag transfected HEK 293 cell lysates
Product Name
His-Tag Polyclonal Antibody
Catalog Number
RD86615A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. It is the most widely used protein affinity tag. Due to its small size of only six amino acids it rarely in
Immunogen Information
Immunogen
Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.
Synonyms
6His epitope tagHexa His tagHHHHHH epitope tagHHHHHH tagHis tagPolyhistidine Tag
Calculated MW
43kDa
Observed MW
43kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.