HIST1H1C Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89550A
Immunohistochemistry of paraffin-embedded human esophageal cancer using HIST1H1C Polyclonal Antibody atdilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using HIST1H1C Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using HIST1H1C Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using HIST1H1C Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
HIST1H1C Polyclonal Antibody
Catalog Number
RD89550A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Immunogen Information
Immunogen
A synthetic peptide of human HIST1H1C
Gene ID
3006
Swissprot
P16403
Synonyms
HIST1H1CH1.2H1CH1F2H1s-1
Calculated MW
47kDa/53kDa
Observed MW
65KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas