HIST1H3A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD253536A
Western blot analysis of 293T and NIH/3T3 cell Mouse liver tissue HUVEC cell lysates using HIST1H3A Polyclonal Antibody at dilution of 1:250
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using HIST1H3A Polyclonal Antibody at dilution of 1:30(×200)
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using HIST1H3A Polyclonal Antibody at dilution of 1:30(×200)
Product Name
HIST1H3A Polyclonal Antibody
Catalog Number
RD253536A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Immunogen Information
Immunogen
Synthetic peptide of human HIST1H3A
Swissprot
P68431
Synonyms
H3 histone familymember AH3/AH31H3FAHist1h3aHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3Jhistone 1H3aHistone cluster 1H3aHistone H3.1Histone H3/aHistone H3/bHistone H3/cHistone H3/dHistone H3/fHistone H3
Calculated MW
15 kDa
Observed MW
Refer to figures
Gene Accession
NP003520
Applications
Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas