Histone H2AX Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD267407A
Immunofluorescence analysis of U-2 OS cells using Histone H2AX Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Product Name
Histone H2AX Polyclonal Antibody
Catalog Number
RD267407A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Immunogen Information
Immunogen
A synthetic peptide of human Histone H2AX.
Gene ID
3014
Swissprot
P16104
Synonyms
H2A.XH2A/XH2AXHistone H2AXH2AFXhistone H2AXgamma H2A.XγH2AX
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IF
Conjugation
Unconjugated
Dilution
IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas