Histone H3.3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD81614A
Western blot analysis of extracts of various cell lines using Histone H3. 3 Polyclonal Antibody at dilution of 1:3000.
Product Name
Histone H3.3 Polyclonal Antibody
Catalog Number
RD81614A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
Immunogen Information
Immunogen
Recombinant fusion protein of human H3F3A (NP_002098.1).
Gene ID
3020
Swissprot
P84243
Synonyms
H3F3AH3.3AH3F3histone H3.3
Calculated MW
15 kDa
Observed MW
16 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas