Histone H3 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western Blot analysis of 1) Hela, 2) Raw264.7, 3) Mouse brain, 4) Rat brain using Histone H3 Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded Human uterus tissue using Histone H3 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat liver tissue using Histone H3 Monoclonal Antibody at dilution of 1:200.
Product Name
Histone H3 Monoclonal Antibody
Catalog Number
RD85461A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Immunogen Information
Immunogen
Recombinant Protein
Swissprot
P68431,Q71DI3,P84243
Synonyms
HIST1H3AH3FAHIST1H3BH3FLHIST1H3CH3FCHIST1H3DH3FBHIST1H3EH3FDHIST1H3FH3FIHIST1H3GH3FHHIST1H3HH3FKHIST1H3IH3FFHIST1H3JH3FJHistone H3.1Histone H3/aHistone H3/bHistone H3/cHistone H3/dHistone H3/fHistone H3/
Calculated MW
15 kDa
Observed MW
15 kDa
Applications
Reactivity
Human,Mouse,Rat,Yeast
Tested Applications
WB,IHC-p,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-300, IF 1:100-500, IP 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas