Histone H3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot of Mouse spleen lysates with anti-Histone H3 pAb at dilution of 1:1000.
Western Blotting with anti-Histone H3 polyclonal antibody at dilution of 1:5000.
Lane 1:HT-29 cell lysates, Lane 2: NIH/3T3 cell lysates, Lane 3: C6
Product Name
Histone H3 Polyclonal Antibody
Catalog Number
RD86628A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. This antibody is not cross-reactive with other histones.
Immunogen Information
Immunogen
Synthetic peptide derived from N-terminus of human Histone H3 protein conjugated to keyhole limpet haemocyanin.
Gene ID
8350
Swissprot
P68431
Synonyms
H3 histone familymember AH3/AH31H3FAHist1h3aHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3Jhistone 1H3aHistone cluster 1H3aHistone H3.1Histone H3/aHistone H3/bHistone H3/cHistone H3/d
Calculated MW
21kDa
Observed MW
21kDa, 50kDa
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000
Concentration
2.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas