Histone H4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western Blot analysis of K562 cell, Mouse pancreas tissue and Hela cell, Mouse thymus tissue and 293T cell, NIH/3T3 and LoVo cell using Histone H4 Polyclonal Antibody at dilution of 1:300
Immunohistochemistry of paraffin-embedded Human colon cancer using Histone H4 Polyclonal Antibody at dilution of 1:30
Immunohistochemistry of paraffin-embedded Human liver cancer using Histone H4 Polyclonal Antibody at dilution of 1:30
Product Name
Histone H4 Polyclonal Antibody
Catalog Number
RD75884A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Immunogen Information
Immunogen
Recombinant protein of human HIST4H4
Swissprot
P62805
Synonyms
H4H4F2H4FNHIST1H4HIST2H4Hist4h4HISTH4H4Histone H4methyl histone H4methylated histone H4
Calculated MW
11 kDa
Gene Accession
NP_003529
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200
Concentration
0.3 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas