HLA-DRB5 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD87518A
Western blot analysis of extracts of various cell lines using HLA-DRB5 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using HLA-DRB5 Polyclonal Antibody at 1:3000 dilution.
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Overview

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Product Name
HLA-DRB5 Polyclonal Antibody
Catalog Number
RD87518A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Immunogen Information

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Immunogen
Recombinant fusion protein of human HLA-DRB5
Gene ID
3127
Swissprot
Q30154
Synonyms
HLA-DRB5HLA-DRBmajor histocompatibility complexclass IIDR beta 5
Calculated MW
37kDa
Observed MW
38kDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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