HLA-E Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD87800A
Western blot analysis of extracts of various cell lines using HLA-E Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using HLA-E Polyclonal Antibody at 1:1000 dilution.
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Overview

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Product Name
HLA-E Polyclonal Antibody
Catalog Number
RD87800A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

Immunogen Information

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Immunogen
Recombinant fusion protein of human HLA-E
Gene ID
3133
Swissprot
P13747
Synonyms
HLA-6.2QA1HLA-EEA1.2EA2.1
Calculated MW
40kDa
Observed MW
40kDa

Applications

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Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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