HLA-F Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD265765A
Western blot analysis of extracts of A-549 cells using HLA-F Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of A-549 cells using HLA-F Polyclonal Antibody at dilution of 1:1000.
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Overview

Circle
Product Name
HLA-F Polyclonal Antibody
Catalog Number
RD265765A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human HLA-F (NP_001091949.1).
Gene ID
3134
Swissprot
P30511
Synonyms
HLA-FCDA12HLA-5.4HLA-CDA12HLAF
Calculated MW
28 kDa/39 kDa/50 kDa
Observed MW
50 kDa

Applications

Circle
Reactivity
Human,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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