For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD78541A
Western Blot analysis of Human hepatocellular carcinoma tissue, Hela and Jurkat cell, 293T cell and Human breast infiltRative duct tissue using HMGB1 Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human thyroid cancer using HMGB1 Polyclonal Antibody at dilution of 1:30
High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
Immunogen Information
Immunogen
Synthetic peptide of human HMGB1
Swissprot
P09429
Synonyms
AmphoterinChromosomal proteinnonhistoneHMG1DKFZp686A04236High mobility group 1High mobility group box 1High mobility group protein 1High mobility group protein B1high-mobility group (nonhistone chromosomal) protein 1HMG-1HMG1HMG3HMGB 1HMGB1
Calculated MW
25 kDa
Gene Accession
NP_002119
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200
Concentration
0.3 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4