HN1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88477A
Western blot analysis of extracts of HT-29 cells using HN1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Rat testis using HN1 Polyclonal Antibody at dilution of 1:150 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse kidney using HN1 Polyclonal Antibody at dilution of 1:100 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human tonsil using HN1 Polyclonal Antibody at dilution of 1:100 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
HN1 Polyclonal Antibody
Catalog Number
RD88477A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Modulates negatively AKT-mediated GSK3B signaling. Induces CTNNB1 'Ser-33' phosphorylation and degradation through the suppression of the inhibitory 'Ser-9' phosphorylation of GSK3B, which represses the function of the APC:CTNNB1:GSK3B complex and the interaction with CDH1/E-cadherin in adherent junctions. Plays a role in the regulation of cell cycle and cell adhesion. Has an inhibitory role on AR-signaling pathway through the induction of receptor proteosomal degradation.
Immunogen Information
Immunogen
Recombinant fusion protein of human HN1
Gene ID
51155
Swissprot
Q9UK76
Synonyms
JPT1ARM2HN1HN1A
Calculated MW
22kDa
Observed MW
23kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.