For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88757A
Western blot analysis of extracts of various cell lines using HSD17B7 Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human breast cancer using HSD17B7 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
HSD17B7 encodes an enzyme that functions both as a 17-beta-hydroxysteroid dehydrogenase (EC 1.1.1.62) in the biosynthesis of sex steroids and as a 3-ketosteroid reductase (EC 1.1.1.270) in the biosynthesis of cholesterol (Marijanovic et al., 2003 [PubMed 12829805]).
Immunogen Information
Immunogen
Recombinant fusion protein of human HSD17B7
Gene ID
51478
Swissprot
P56937
Synonyms
HSD17B7PRAPSDR37C1
Calculated MW
31kDa
Observed MW
45KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.