HSPA5 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90660A
Western blot with HSPA5 Polyclonal antibody at dilution of 1:500.lane 1：Hela whole cell lysate,lane 2：Jurkat whole cell lysate,lane 3：THP-1 whole cell lysate,lane 4：HT-29 whole cell lysate,lane 5:NIH/3T3 whole cell lysate,lane 6:RAW264.7 whole cell lysate,lane 7:Mouse lung
Product Name
HSPA5 Polyclonal Antibody
Catalog Number
RD90660A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen.Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate .Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR).In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1.
Immunogen Information
Immunogen
Recombinant Human HSPA5 protein expressed by E.coli
Swissprot
P11021
Synonyms
HSPA5GRP7878 kDa glucose-regulated proteinBinding-immunoglobulin proteinHeat shock protein 70 family protein 5Heat shock protein family A member 5Immunoglobulin heavy chain-binding protein
Calculated MW
25 kDa
Observed MW
27 kDa
Applications
Reactivity
Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas