HTRA2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD88030A
Western blot analysis of extracts of various cell lines using HTRA2 Polyclonal Antibody at1:1000 dilution.
Western blot analysis of extracts of various cell lines using HTRA2 Polyclonal Antibody at1:1000 dilution.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3 ug HTRA2 Polyclonal Antibody.Western blot was performed from the immunoprecipitate using HTRA2 Polyclonal Antibody at a dilution of 1:1000.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3 ug HTRA2 Polyclonal Antibody.Western blot was performed from the immunoprecipitate using HTRA2 Polyclonal Antibody at a dilution of 1:1000.
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Overview

Circle
Product Name
HTRA2 Polyclonal Antibody
Catalog Number
RD88030A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human HTRA2
Gene ID
27429
Swissprot
O43464
Synonyms
HTRA2MGCA8OMIPARK13PRSS25
Calculated MW
21kDa
Observed MW
22kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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