For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88836A
Western blot analysis of extracts of various cell lines using ICA1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human colon using ICA1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
Immunogen Information
Immunogen
Recombinant fusion protein of human ICA1
Gene ID
3382
Swissprot
Q05084
Synonyms
ICA69ICAp69ICA1
Calculated MW
51kDa
Observed MW
55kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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