For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86674A
Western blotting with anti-ICAM-1 polyclonal antibody at dilution of 1:1000. Lane 1: Rat kidney lysate, Lane 2: Rat spleen lysate, lane 3: Rat liver lysate.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Immunogen Information
Immunogen
Recombinant Rat ICAM-1 protein expressed by E.coli.
Gene ID
25464
Swissprot
Q00238
Synonyms
Icam1intercellular adhesion molecule 1CD54
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
2.2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.