For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89095A
Western blot analysis of extracts of various cell lines using IER5 Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human esophageal using IER5 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat pancreas using IER5 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a protein that is similar to other immediate early response proteins. In the mouse, a similar gene may play an important role in mediating the cellular response to mitogenic signals. Studies in rats found the expression of a similar gene to be increased after waking and sleep deprivation. [provided by RefSeq, Jul 2008]
Immunogen Information
Immunogen
A synthetic peptide of human IER5
Gene ID
51278
Swissprot
Q5VY09
Synonyms
SBBI48IER5
Observed MW
67KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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