For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90163A
Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug IKKε Polyclonal Antibody. Western blot was performed from the immunoprecipitate using IKKε Polyclonal Antibody at a dilution of 1:1000.
IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines.
Immunogen Information
Immunogen
Recombinant fusion protein of human IKKε
Gene ID
9641
Swissprot
Q14164
Synonyms
IKBKEIKK-EIKK-iIKKEIKKI
Calculated MW
51kDa/52kDa/56kDa/59kDa
Observed MW
70KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.