For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88692A
Western blot analysis of extracts of Jurkat cells using IL2 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours
Western blot analysis of extracts of Recombinant Human IL2 Protein using IL2 Polyclonal Antibody at 1:1000 dilution.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Immunogen Information
Immunogen
Recombinant fusion protein of human IL2
Gene ID
3558
Swissprot
P60568
Synonyms
IL2IL-2TCGFlymphokine
Calculated MW
52kDa/54kDa/55kDa/60kDa/63kDa/64kDa
Observed MW
55kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.