For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90239A
Western blot analysis of extracts of RAW264.7 cells using iNOS Polyclonal Antibody at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Immunogen Information
Immunogen
Recombinant fusion protein of human iNOS
Gene ID
4843
Swissprot
P35228
Synonyms
NOSINOSNOS2AHEP-NOSiNOSNOS2
Calculated MW
31kDa
Observed MW
31KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.