INSIG2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89200A
Western blot analysis of extracts of Mouse kidney using INSIG2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using INSIG2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse brain using INSIG2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat ovary using INSIG2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
INSIG2 Polyclonal Antibody
Catalog Number
RD89200A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.
Immunogen Information
Immunogen
A synthetic peptide of human INSIG2
Gene ID
51141
Swissprot
Q9Y5U4
Synonyms
INSIG2INSIG-2
Calculated MW
56kDa
Observed MW
57KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas