For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89277A
Western blot analysis of extracts of various cell lines using INSM1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human small cell lung cancer using INSM1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Insulinoma-associated 1 (INSM1) gene is intronless and encodes a protein containing both a zinc finger DNA-binding domain and a putative prohormone domain. This gene is a sensitive marker for neuroendocrine differentiation of human lung tumors.
Immunogen Information
Immunogen
Recombinant fusion protein of human INSM1
Gene ID
3642
Swissprot
Q01101
Synonyms
IA1IA-1
Calculated MW
97kDa
Observed MW
100KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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