ITCH Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88819A
Western blot analysis of extracts of 293T cells using ITCH Polyclonal Antibody at 1:500 dilution.
Western blot analysis of extracts of various cell lines using ITCH Polyclonal Antibody at dilution.
Immunohistochemistry of paraffin-embedded rat brain using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
ITCH Polyclonal Antibody
Catalog Number
RD88819A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human ITCH
Gene ID
83737
Swissprot
Q96J02
Synonyms
ITCHADMFDAIF4AIP4NAPP1
Calculated MW
39kDa/53kDa/56kDa/61kDa
Observed MW
57kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas