For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89499A
Western blot analysis of extracts of HeLa cells using KDM3B Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using KDM3B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
Immunogen Information
Immunogen
Recombinant fusion protein of human KDM3B
Gene ID
51780
Swissprot
Q7LBC6
Synonyms
KDM3B5qNCAC5orf7JMJD1BNET22
Calculated MW
32kDa/41kDa
Observed MW
42kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.