For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89789A
Western blot analysis of extracts of various cell lines using KDM5A Polyclonal Antibody at 1:500 dilution.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A Polyclonal Antibody.Western blot was performed from the immunoprecipitate using KDM5A Polyclonal Antibody at a dilution of 1:500.
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
Immunogen Information
Immunogen
Recombinant fusion protein of human KDM5A
Gene ID
5927
Swissprot
P29375
Synonyms
KDM5ARBBP-2RBBP2RBP2
Calculated MW
109kDa
Observed MW
109kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.