For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87716A
Western blot analysis of extracts of various cell lines using KRT36 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using KRT36 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.
Immunogen Information
Immunogen
A synthetic peptide of human KRT36
Gene ID
8689
Swissprot
O76013
Synonyms
KRT36HA6KRTHA6hHa6
Calculated MW
31kDa/54kDa
Observed MW
50kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.