KU70 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80148A
Western blot analysis of extracts of various cell lines using KU70 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using KU70 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human breast using KU70 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human leiomyoma of uterus using KU70 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse lung using KU70 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using KU70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using KU70 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
KU70 Polyclonal Antibody
Catalog Number
RD80148A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
Immunogen Information
Immunogen
A synthetic peptide of human KU70 (NP_001460.1).
Gene ID
2547
Swissprot
P12956
Synonyms
XRCC6CTC75CTCBFG22P1KU70ML8TLAA
Calculated MW
65 kDa/69 kDa
Observed MW
70 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas