Lamin A/C Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80004A
Western blot analysis of extracts of various cell lines using Lamin A/C Polyclonal Antibody at dilution of 1:1000. HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM) at 37°C for 20 hours.
Immunohistochemistry of paraffin-embedded Rat brain using Lamin A/C Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using Lamin A/C Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).
Confocal immunofluorescence analysis of A-431 cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody
Product Name
Lamin A/C Polyclonal Antibody
Catalog Number
RD80004A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Immunogen Information
Immunogen
Recombinant fusion protein of human Lamin C (NP_005563.1).
Gene ID
4000
Swissprot
P02545
Synonyms
LMNACDCD1CDDCCMD1ACMT2B1EMD2FPLFPLDFPLD2HGPSIDCLDP1LFPLGMD1BLMN1LMNCLMNL1MADAPRO1laminLamin A/C
Calculated MW
62-74 kDa
Observed MW
69 kDa/72 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas