LC3B Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD80562A
Western blot analysis of extracts of various cell lines using LC3B Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using LC3B Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using LC3B Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using LC3B Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using LC3B Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using LC3B Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of LC3B knockout 293T cells treated by chloroquine (top left) and wild-type 293T cells (top middle),wild-type 293T cells treated by chloroquine (top right) using LC3B Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of LC3B knockout 293T cells treated by chloroquine (top left) and wild-type 293T cells (top middle),wild-type 293T cells treated by chloroquine (top right) using LC3B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
LC3B Polyclonal Antibody
Catalog Number
RD80562A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Immunogen Information

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Immunogen
A synthetic peptide of human LC3B (NP_073729.1).
Gene ID
81631
Swissprot
Q9GZQ8
Synonyms
LC3BATG8FMAP1LC3B-aMAP1A/1BLC3MAP1LC3BLC3A/LC3B
Calculated MW
14 kDa
Observed MW
14 kDa/15 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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