LC3B Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86837A
Western blot analysis of extracts of various lysates using LC3B Polyclonal Antibody at 1:2000 dilution.NIH/3T3 and C6 were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Immunohistochemistry of paraffin-embedded Human breast cancer using LC3B Polyclonal Antibody at dilution of 1:250 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
LC3B Polyclonal Antibody
Catalog Number
RD86837A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Immunogen Information
Immunogen
A synthetic peptide of human LC3B
Gene ID
81631
Swissprot
Q9GZQ8
Synonyms
LC3BATG8FMAP1LC3B-aMAP1A/1BLC3MAP1LC3BLC3A/LC3B
Calculated MW
10kDa
Observed MW
12KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.