LDHA Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90692A
Western blot with LDHA Polyclonal antibody at dilution of 1:2500.lane 1:Hela whole cell lysate,lane 2：NIH/3T3 whole cell lysate,lane 3：Mouse kidney,lane 4：Mouse heart,lane 5：Mouse skeletal muscle,lane 6：Rat kidney,lane 7：Rat heart,lane 8：Rat skeletal muscle
Immunohistochemistry of paraffin-embedded Human liver cancer using LDHA Polyclonal Antibody at dilution of 1:400
Product Name
LDHA Polyclonal Antibody
Catalog Number
RD90692A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
LDHA,also named as LDH-M and NY-REN-59,is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues,though predominantly in muscle tissue and tumours,and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development,invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.
Immunogen Information
Immunogen
Recombinant Human LDHA protein expressed by E.coli
Swissprot
P00338
Synonyms
Cell proliferation-inducing gene 19 proteinGSD11L lactate dehydrogenase A chainl7R2LDH MLDH muscle subunitLDH1PIG19 Proliferation-inducing gene 19
Calculated MW
23kDa
Observed MW
25kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:2500-1:5000, IHC 1:200-1:400
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas