LDHA Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD219937A
Western blot analysis of A375 and Hela cell lysates  using LDHA Polyclonal Antibody at dilution of 1:400
Western blot analysis of A375 and Hela cell lysates using LDHA Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human breast cancer tissue  using LDHA Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using LDHA Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue  using LDHA Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using LDHA Polyclonal Antibody at dilution of 1:40(×200)
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Overview

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Product Name
LDHA Polyclonal Antibody
Catalog Number
RD219937A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

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LDHA,also named as LDH-M and NY-REN-59,is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues,though predominantly in muscle tissue and tumours,and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development,invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.

Immunogen Information

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Immunogen
Synthetic peptide of human LDHA
Swissprot
P00338
Synonyms
Cell proliferation-inducing gene 19 proteinL lactate dehydrogenase A chainLactate dehydrogenase ALDH ALDH MLDH muscle subunitLDH1LDH5LDHAPIG 19PIG19Renal carcinoma antigen NY-REN-59
Calculated MW
37 kDa
Observed MW
Refer to figures
Gene Accession
NP005557

Applications

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Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:30-1:150, ELISA 1:5000-1:10000
Concentration
1.14 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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