LIG4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD218863A
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using LIG4 Polyclonal Antibody at dilution of 1:40(×200)
Product Name
LIG4 Polyclonal Antibody
Catalog Number
RD218863A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Immunogen Information
Immunogen
Fusion protein of human LIG4
Swissprot
P49917
Synonyms
DNA ligase IV ATP dependentDNA joinaseDNA ligase 4DNA ligase IVDNA repair enzymeDNLI4LIG 4LIG4LIG4SLigase IVLigase IV DNA ATP dependentPolydeoxyribonucleotide synthase [ATP] 4Polydeoxyribonucleotide synthase 4Polydeoxyribonucleotide synthase
Gene Accession
BC037491
Applications
Reactivity
Human, Mouse
Tested Applications
IHC,ELISA
Conjugation
Unconjugated
Dilution
IHC 1:30-1:150, ELISA 1:5000-1:10000
Concentration
0.72 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas