LLGL1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89234A
Western blot analysis of extracts of various cell lines using LLGL1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat testis using LLGL1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver cancer using LLGL1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using LLGL1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-549 cells using LLGL1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using LLGL1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
LLGL1 Polyclonal Antibody
Catalog Number
RD89234A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Immunogen Information
Immunogen
Recombinant fusion protein of human LLGL1
Gene ID
3996
Swissprot
Q15334
Synonyms
DLG4HUGLLLGLLgl1Mgl1HUGL1HUGL-1
Calculated MW
37kDa/39kDa
Observed MW
40KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas