For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89234A
Western blot analysis of extracts of various cell lines using LLGL1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat testis using LLGL1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver cancer using LLGL1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using LLGL1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-549 cells using LLGL1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using LLGL1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
This gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Immunogen Information
Immunogen
Recombinant fusion protein of human LLGL1
Gene ID
3996
Swissprot
Q15334
Synonyms
DLG4HUGLLLGLLgl1Mgl1HUGL1HUGL-1
Calculated MW
37kDa/39kDa
Observed MW
40KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.