Luciferase Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85555A
Western Blot analysis of Luciferase protein using Luciferase Monoclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of Mouse heart tissue using Luciferase Monoclonal Antibody at dilution of 1:200.
Product Name
Luciferase Monoclonal Antibody
Catalog Number
RD85555A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Immunogen Information
Immunogen
Recombinant Protein of Luciferase of Luciferase
Swissprot
P08659
Synonyms
ec 1.13.12.7FireflyLuciferaseLuciferin 4 monooxygenase
Observed MW
60 kDa
Applications
Reactivity
Firefly
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas