For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86802A
Western blot analysis of various lysates using MAP2 Polyclonal Antibody at 1:2000 dilution.
Immunohistochemistry of paraffin-embedded Rat brain using MAP2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat brain using MAP2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse brain using MAP2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse brain using MAP2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of mouse brain cells using MAP2 Polyclonal antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain cells using MAP2 Polyclonal antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
Immunogen Information
Immunogen
Recombinant protein of human Map2.
Gene ID
4133
Swissprot
P11137
Synonyms
MAP2MAP2AMAP2BMAP2CMap2
Calculated MW
24kDa/33kDa
Observed MW
34kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.