MAP2K4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89433A
Western blot analysis of A-549 using MAP2K4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of various lysates using MAP2K4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse liver using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat ovary using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse kidney using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human colon cancer using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat lung using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
MAP2K4 Polyclonal Antibody
Catalog Number
RD89433A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human MAP2K4
Gene ID
6416
Swissprot
P45985
Synonyms
MAP2K4JNKKJNKK1MAPKK4MEK4MKK4PRKMK4SAPKK-1SAPKK1SEK1SERK1SKK1mitogen-activated protein kinase kinase 4
Calculated MW
84kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas