Western blot analysis of extracts of various cell lines using MAPKAP1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat kidney using MAPKAP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human MAPKAP1
Gene ID
79109
Swissprot
Q9BPZ7
Synonyms
MAPKAP1JC310MIP1SIN1SIN1bSIN1g
Calculated MW
31kDa/101kDa/107kDa/113kDa/114kDa/120kDa
Observed MW
120kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.