MCAM Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD253284A
Western blot analysis of Human leiomyosarcoma tissue lysate using MCAM Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MCAM Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using MCAM Polyclonal Antibody at dilution of 1:40(×200)
Product Name
MCAM Polyclonal Antibody
Catalog Number
RD253284A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
CD146,also known as melanoma cell adhesion molecule (MCAM) or MUC18,originally identified as a biomarker of melanoma progression,is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily . Structurally,it consists of five Ig domains,a transmembrane domain,and a cytoplasmic region. In normal adult tissue,CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis,and has been used as marker of circulating endothelium cells (CECs) .
Immunogen Information
Immunogen
Synthetic peptide of human MCAM
Swissprot
P43121
Synonyms
A32 antigenCD 146CD146CD146 antigenCell surface glycoprotein MUC18Cell surface glycoprotein P1H12GicerinMcamMelanoma adhesion moleculeMelanoma associated antigen A32Melanoma associated antigen MUC18Melanoma associated glycoprotein MUC18Melanom
Calculated MW
72 kDa
Observed MW
Refer to figures
Gene Accession
NP006491
Applications
Reactivity
Human
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:40-1:200, ELISA 1:5000-1:10000
Concentration
1.14 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas