For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86617A
Western blotting of mCherry transfected HEK293 cell with Anti-mcherry polyclonal antibody at dilution of 1:2000.
Lane 1: 10ul cell lysate, Lane 2: 5ul cell lysates.
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish.
Immunogen Information
Immunogen
Recombinant Discosoma sp. mCherry Protein
Swissprot
X5DSL3
Synonyms
mCherry-Tag
Calculated MW
20kDa
Observed MW
20kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
1.2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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