MCM10 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD89788A
Western blot analysis of extracts of Jurkat cells using MCM10 Polyclonal Antibody at 1:500 dilution.
Western blot analysis of extracts of Jurkat cells using MCM10 Polyclonal Antibody at 1:500 dilution.
Variation
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Overview
Circle
Product Name
MCM10 Polyclonal Antibody
Catalog Number
RD89788A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Circle
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Immunogen Information
Circle
Immunogen
Recombinant fusion protein of human MCM10
Gene ID
55388
Swissprot
Q7L590
Synonyms
MCM10CNA43DNA43PRO2249
Calculated MW
186kDa/192kDa
Observed MW
192kDa
Applications
Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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